Simple Plex Human Chitinase 3-like 1/YKL-40 Cartridge

R&D Systems | Catalog # SPCKB-PS-000255

Simple Plex Human Chitinase 3-like 1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

6.68 pg/mL

Assay Range

6.68-25500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Simple Plex Human Chitinase 3-like 1/YKL-40 Cartridge Summary

Simple Plex assay for the detection of human Chitinase 3-like 1 (CHI3L1) in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Chitinase 3-like 1/YKL-40 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CHI3L1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 22
Mean (pg/mL) 341 30384 373 26287
Standard Deviation 23.5 1905 35.8 2724
CV% 6.9 6.3 9.6 10.4

Recovery for Simple Plex Human Chitinase 3-like 1/YKL-40 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 80 75-84

Linearity

Samples containing and/or spiked with high concentrations of human CHI3L1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Chitinase 3-like 1 Ella Assay Linearity

Scientific Data Images for Simple Plex Human Chitinase 3-like 1/YKL-40 Cartridge

Human Chitinase 3-like 1 Ella Assay Standard Curve

Human Chitinase 3-like 1 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1/YKL-40 (CHI3L1), also called BRP39 in mouse, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Alternate Names

CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Gene Symbol

CHI3L1

Additional Chitinase 3-like 1/YKL-40 Products

Product Documents for Simple Plex Human Chitinase 3-like 1/YKL-40 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Chitinase 3-like 1/YKL-40 Cartridge

For research use only

Citations for Simple Plex Human Chitinase 3-like 1/YKL-40 Cartridge

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