Simple Plex Human Clusterin Cartridge

R&D Systems | Catalog # SPCKB-PS-001512

Simple Plex Human Clusterin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

6.14 pg/mL

Assay Range

40.5-386400 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human Clusterin Cartridge Summary

Simple Plex assay is for the detection of human Clusterin in serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Clusterin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Clusterin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Clusterin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Clusterin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Clusterin.

EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 44 43
Mean (pg/mL) 2303 103,870 2317 111,162
Standard Deviation 101 4562 260 8163
CV% 4.4 4.4 11.2 7.3

Recovery for Simple Plex Human Clusterin Cartridge

Recovery was not necessary as human Clusterin shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Clusterin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Clusterin Assay Linearity

Scientific Data Images for Simple Plex Human Clusterin Cartridge

Simple Plex Human Clusterin Assay Standard Curve

Simple Plex Human Clusterin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Clusterin Assay. Assay range is 40.5-386,400 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Clusterin

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a secreted multifunctional protein that binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis. Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development. It is secreted as a disulfide-linked heterodimer of alpha and beta chains and circulates as a component of high density lipoprotein (HDL) particles. Clusterin exerts tissue protective functions in atherosclerosis and Alzheimer’s disease by binding and neutralizing non-oxidatively modified LDL reduces cytotoxicity and beta-amyloid fibrils, respectively. Increased circulating levels of Clusterin enhance tumor aggressiveness by inhibiting apoptosis and by promoting epithelial to mesenchymal transition.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Entrez Gene IDs

1191 (Human); 12759 (Mouse)

Gene Symbol

CLU

Additional Clusterin Products

Product Documents for Simple Plex Human Clusterin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Clusterin Cartridge

For research use only

Citations for Simple Plex Human Clusterin Cartridge

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