Simple Plex Human COMP Cartridge

R&D Systems | Catalog # SPCKB-PS-000756

Simple Plex Human COMP/Thrombospondin-5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.93 pg/mL

Assay Range

8.44-32196 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human COMP Cartridge Summary

Simple Plex assay is for the detection of human Cartilage Oligomeric Matrix Protein (COMP) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all COMP/Thrombospondin-5 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human COMP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one COMP assay.

Inter-Assay Precision (Precision between assays) Replicates of each COMP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human COMP.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 24 24
Mean (pg/mL) 234 11010 250 12439
Standard Deviation 17.7 847 32.6 983
CV% 7.6 7.7 13.1 7.9

Recovery for Simple Plex Human COMP Cartridge

Recovery for the human COMP assays was evaluated at three different spiked concentrations of CD40 protein.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 103 97-111

Linearity

Samples containing and/or spiked with high concentrations of human COMP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human COMP Assay Linearity

Scientific Data Images for Simple Plex Human COMP Cartridge

Simple Plex Human COMP Assay Standard Curve

Simple Plex Human COMP Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human COMP Assay. Assay range is 8.44-32,196 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: COMP/Thrombospondin-5

COMP (Cartilage Oligomeric Matrix Protein), also known as Thrombospondin-5, is a multidomain calcium binding protein that associates with other extracellular matrix molecules. COMP associates into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands. An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrins a5b1 or aVb3. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Several mutations in COMP are associated with skeletal dysplasias.

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Entrez Gene IDs

1311 (Human); 12845 (Mouse)

Gene Symbol

COMP

Additional COMP/Thrombospondin-5 Products

Product Documents for Simple Plex Human COMP Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human COMP Cartridge

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Simple Plex Human COMP Cartridge

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