Simple Plex Human COMP Cartridge

Catalog #: SPCKB-PS-000756 Datasheet / COA / SDS

Simple Plex Human COMP/Thrombospondin-5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000756
SPCKA-PS-000756
SPCKC-PS-000756

Control Products Available

898620: Simple Plex Control for Human COMP/Thrombospondin-5

Ella Automated ELISA Platform and Simple Plex Human COMP Assay

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All COMP/Thrombospondin-5 Simple Plex Assays

Product Details

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human COMP Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.93 pg/mL

Assay Range

8.4 - 32,196 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human COMP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Cartilage Oligomeric Matrix Protein (COMP) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one COMP assay

Inter-Assay Precision (Precision between assays) Replicates of each COMP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human COMP

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	24
Mean (pg/mL)	234	11010	250	12439
Standard Deviation	17.7	847	32.6	983
CV%	7.6	7.7	13.1	7.9

Recovery

Recovery for the human COMP assays was evaluated at three different spiked concentrations of CD40 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	103	97-111

Linearity

Samples containing and/or spiked with high concentrations of human COMP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human COMP Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human COMP Assay. Assay range is 8.44-32,196 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: COMP/Thrombospondin-5

COMP (Cartilage Oligomeric Matrix Protein), also known as Thrombospondin-5, is a multidomain calcium binding protein that associates with other extracellular matrix molecules. COMP associates into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands. An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrins a5b1 or aVb3. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Several mutations in COMP are associated with skeletal dysplasias.

Long Name:

Cartilage Oligomeric Matrix Protein

Entrez Gene IDs:

1311 (Human); 12845 (Mouse)

Alternate Names:

cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5