Simple Plex Human CTGF/CCN2 Cartridge

Catalog #: SPCKB-PS-000755 Datasheet / COA / SDS

Simple Plex Human CTGF/CCN2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000755
SPCKA-PS-000755
SPCKC-PS-000755

Control Products Available

898495: Simple Plex Control for Human CTGF/CCN2

Ella Automated ELISA Platform and Simple Plex Human CTGF Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CTGF/CCN2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

4.23 pg/mL

Assay Range

17.0 - 65,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human CTGF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Connective Tissue Growth Factor/CCN2 (CTGF) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CTGF assay

Inter-Assay Precision (Precision between assays) Replicates of each CTGF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CTGF

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	17
Mean (pg/mL)	401	20658	376	17942
Standard Deviation	31.9	1330	25.4	1608
CV%	7.9	6.4	6.7	9

Recovery

Recovery for the human CTGF assays was evaluated at three different spiked concentrations of CTGF protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	88	86-90
Heparin Plasma (n=4)	86	83-89

Linearity

Samples containing and/or spiked with high concentrations of human CTGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human CTGF Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CTGF Assay. Assay range is 17.0-65,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CTGF/CCN2

CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contribute to the adhesive and mitogenic properties of CTGF.

Long Name:

Connective Tissue Growth Factor

Entrez Gene IDs:

1490 (Human); 14219 (Mouse); 64032 (Rat)

Alternate Names:

CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGF; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2