Simple Plex Human CTGF/CCN2 Cartridge

R&D Systems | Catalog # SPCKB-PS-000755

Simple Plex Human CTGF/CCN2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

4.23 pg/mL

Assay Range

17-65000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CTGF/CCN2 Cartridge Summary

Simple Plex assay is for the detection of human Connective Tissue Growth Factor/CCN2 (CTGF) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CTGF/CCN2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CTGF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CTGF assay.

Inter-Assay Precision (Precision between assays) Replicates of each CTGF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CTGF.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 17 17
Mean (pg/mL) 401 20,658 376 17,942
Standard Deviation 31.9 1330 25.4 1608
CV% 7.9 6.4 6.7 9.0

Recovery for Simple Plex Human CTGF/CCN2 Cartridge

Recovery for the human CTGF assays was evaluated at three different spiked concentrations of CTGF protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 88 86-90
Heparin Plasma (n=4) 86 83-89

Linearity

Samples containing and/or spiked with high concentrations of human CTGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human CTGF Assay Linearity

Scientific Data Images for Simple Plex Human CTGF/CCN2 Cartridge

Simple Plex Human CTGF Assay Standard Curve

Simple Plex Human CTGF Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CTGF Assay. Assay range is 17.0-65,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CTGF/CCN2

CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the HSPG (proteoglycan) and low density lipoprotein receptor (LDLR) binding sites that contribute to the adhesive and mitogenic properties of CTGF.

Long Name

Connective Tissue Growth Factor

Alternate Names

CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2

Entrez Gene IDs

1490 (Human); 14219 (Mouse); 64032 (Rat)

Gene Symbol

CCN2

Additional CTGF/CCN2 Products

Product Documents for Simple Plex Human CTGF/CCN2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human CTGF/CCN2 Cartridge

For research use only

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