Simple Plex Human Cystatin C Cartridge

R&D Systems | Catalog # SPCKB-PS-007569

Simple Plex Human Cystatin C assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (500 nL), Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL), Saliva (25 nL), Urine (500 nL), Cerebrospinal Fluid (25 nL)

Sensitivity

0.92 pg/mL

Assay Range

2.69-4102 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)

Simple Plex Human Cystatin C Cartridge Summary

Simple Plex assay for the detection of human Cystatin C in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, urine, and cerebrospinal fluid (CSF)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Cystatin C

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 36
Mean (pg/mL) 41.7 1847 33.7 1656
Standard Deviation 1.46 115 3.74 141
CV% 3.5 6.2 11.1 8.5

Recovery for Simple Plex Human Cystatin C Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=2) 104 104-104

Linearity

Samples containing and/or spiked with high concentrations of human Cystatin C were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Cystatin C Assay Linearity

Scientific Data Images for Simple Plex Human Cystatin C Cartridge

Simple Plex Human Cystatin C Assay Standard Curve

Simple Plex Human Cystatin C Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Cystatin C Assay. Assay range is 2.69-4102 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cystatin C

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

Alternate Names

ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Gene Symbol

CST3

Additional Cystatin C Products

Product Documents for Simple Plex Human Cystatin C Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Cystatin C Cartridge

For research use only

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