Simple Plex Human Cytokeratin 18 Cartridge
R&D Systems | Catalog # SPCKB-PS-001065
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Cytokeratin 18 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Cytokeratin 18 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Cytokeratin 18 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Cytokeratin 18.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 38 | 40 |
| Mean (pg/mL) | 522 | 33,577 | 473 | 29,354 |
| Standard Deviation | 48.9 | 3085 | 50.0 | 2606 |
| CV% | 9.4 | 9.2 | 10.6 | 8.9 |
Recovery for Simple Plex Human Cytokeratin 18 Cartridge
Recovery for the human Cytokeratin 18 assays was evaluated at three different spiked concentrations of CD40 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 104 | 86-116 |
| Heparin Plasma (n=4) | 100 | 81-111 |
| Serum (n=4) | 105 | 80-115 |
Linearity
Samples containing and/or spiked with high concentrations of human Cytokeratin 18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Cytokeratin 18 Cartridge
Simple Plex Human Cytokeratin 18 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Cytokeratin 18 Assay. Assay range is 141-86,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Cytokeratin 18
Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.
Alternate Names
Gene Symbol
Additional Cytokeratin 18 Products
Product Documents for Simple Plex Human Cytokeratin 18 Cartridge
Product Specific Notices for Simple Plex Human Cytokeratin 18 Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human Cytokeratin 18 Cartridge
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