Simple Plex Human D-dimer Cartridge

R&D Systems | Catalog # SPCKB-PS-001976

Simple Plex Human D-dimer assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)

Sensitivity

36.4 pg/mL

Assay Range

142-540890 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Simple Plex Human D-dimer Cartridge Summary

Simple Plex assay is for the detection of human D-Dimer in cell culture supernatant (CCS), serum, and plasma (Citrate/EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all D-dimer Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human D-Dimer.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one D-Dimer assay.

Inter-Assay Precision (Precision between assays) Replicates of each D-Dimer control were tested in multiple assays performed by at least three technicians using two lots of reagents for human D-Dimer.

Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 34 34
Mean (pg/mL) 3288 174,738 3282 173,674
Standard Deviation 226 8198 267 11,649
CV% 6.9 4.7 8.1 6.7

Recovery for Simple Plex Human D-dimer Cartridge

Recovery was not necessary as human D-Dimer shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human D-Dimer were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human D-Dimer Assay Linearity

Scientific Data Images for Simple Plex Human D-dimer Cartridge

Simple Plex Human D-Dimer Assay Standard Curve

Simple Plex Human D-Dimer Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human D-Dimer Assay. Assay range is 142-540,890 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: D-dimer

A fibrin degradation fragment or product that is produced by the action of plasmin on fibrin in the clot dissolution process.

Long Name

D-Dimer breakdown product of Fibrinogen

Alternate Names

Ddimer, FDP

Additional D-dimer Products

Product Documents for Simple Plex Human D-dimer Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human D-dimer Cartridge

For research use only

Citations for Simple Plex Human D-dimer Cartridge

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