Simple Plex Human DcR3 Cartridge

Catalog #: SPCKB-PS-000876 Datasheet / COA / SDS

Simple Plex Human DcR3/TNFRSF6B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000876
SPCKA-PS-000876
SPCKC-PS-000876

Control Products Available

898616: Simple Plex Control for Human DcR3/TNFRSF6B

Ella Automated ELISA Platform and Simple Plex Human DcR3 Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human DcR3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.17 pg/mL

Assay Range

10.5 - 16,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human DcR3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Decoy Receptor 3 (DcR3)/TNFRSF6B in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one DcR3 assay

Inter-Assay Precision (Precision between assays) Replicates of each DcR3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human DcR3

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	57	56
Mean (pg/mL)	116	4714	114	5031
Standard Deviation	6.78	375	11.6	473
CV%	5.8	8	10.3	9.4

Recovery

Recovery was not necessary as human DcR3 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human DcR3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human DcR3 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human DcR3 Assay. Assay range is 10.5-16,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DcR3/TNFRSF6B

Decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNF RII (29%) and Fas (17%), and is expressed in a variety of different tissues and at high levels in many malignant tumors.

Long Name:

Decoy Receptor 3

Entrez Gene IDs:

8771 (Human); 107126607 (Cynomolgus Monkey)

Alternate Names:

DcR3; DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy