Simple Plex Human DcR3 Cartridge

R&D Systems | Catalog # SPCKB-PS-000876

Simple Plex Human DcR3/TNFRSF6B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

1.17 pg/mL

Assay Range

10.49-16000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human DcR3 Cartridge Summary

Simple Plex assay is for the detection of human Decoy Receptor 3 (DcR3)/TNFRSF6B in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human DcR3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one DcR3 assay.

Inter-Assay Precision (Precision between assays) Replicates of each DcR3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human DcR3.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 57 56
Mean (pg/mL) 116 4714 114 5031
Standard Deviation 6.78 375 11.6 473
CV% 5.8 8.0 10.3 9.4

Recovery for Simple Plex Human DcR3 Cartridge

Recovery was not necessary as human DcR3 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human DcR3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human DcR3 Assay Linearity

Scientific Data Images for Simple Plex Human DcR3 Cartridge

Simple Plex Human DcR3 Assay Standard Curve

Simple Plex Human DcR3 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human DcR3 Assay. Assay range is 10.5-16,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DcR3/TNFRSF6B

Decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNF RII (29%) and Fas (17%), and is expressed in a variety of different tissues and at high levels in many malignant tumors.

Long Name

Decoy Receptor 3

Alternate Names

M68, TNFRSF6B, TR6

Entrez Gene IDs

8771 (Human); 107126607 (Cynomolgus Monkey)

Gene Symbol

TNFRSF6B

Additional DcR3/TNFRSF6B Products

Product Documents for Simple Plex Human DcR3 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human DcR3 Cartridge

For research use only

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