Simple Plex Human E-Selectin/CD62E Cartridge

R&D Systems | Catalog # SPCKB-PS-000457

Simple Plex Human E-Selectin/CD62E assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.09 pg/mL

Assay Range

2.56-24460 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human E-Selectin/CD62E Cartridge Summary

Simple Plex assay is for the detection of human E-Selectin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all E-Selectin/CD62E Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human E-Selectin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one E-Selectin assay.

Inter-Assay Precision (Precision between assays) Replicates of each E-Selectin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human E-Selectin.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 17 19
Mean (pg/mL) 156 7614 140 6802
Standard Deviation 9.73 461 12.0 642
CV% 6.2 6.1 8.6 9.4

Recovery for Simple Plex Human E-Selectin/CD62E Cartridge

Recovery for the human E-Selectin assays was evaluated at three different spiked concentrations of E-Selectin protein.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 104 96-109

Linearity

Samples containing and/or spiked with high concentrations of human E-Selectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human E-Selectin Assay Linearity

Scientific Data Images for Simple Plex Human E-Selectin/CD62E Cartridge

Simple Plex Human E-Selectin Assay Standard Curve

Simple Plex Human E-Selectin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human E-Selectin Assay. Assay range is 2.56-24,460 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Selectin/CD62E

E-Selectin, also known as CD62E, is expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. It is a lectin that binds to sialylated or fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells. E-Selectin mediates the initial attachment of circulating leukocytes to the blood vessel wall during inflammation. This interaction permits leukocytes to roll along the vascular endothelium. Subsequent ICAM-1 and VCAM-1 mediated adhesion leads to extravasation of the leukocyte through the blood vessel wall into the extracellular matrix tissue.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for Simple Plex Human E-Selectin/CD62E Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human E-Selectin/CD62E Cartridge

For research use only

Citations for Simple Plex Human E-Selectin/CD62E Cartridge

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