Simple Plex Human FAP Cartridge

Catalog #: SPCKB-PS-000769 Datasheet / COA / SDS

Simple Plex Human Fibroblast Activation Protein alpha/FAP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000769
SPCKA-PS-000769
SPCKC-PS-000769

Control Products Available

898618: Simple Plex Control for Human FAP

Ella Automated ELISA Platform and Simple Plex Human FAP Assay

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All Fibroblast Activation Protein alpha/FAP Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human FAP Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.9 pg/mL

Assay Range

10.5 - 40,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human FAP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Fibroblast Activation Protein alpha (FAP) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FAP assay

Inter-Assay Precision (Precision between assays) Replicates of each FAP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FAP

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	17
Mean (pg/mL)	227	10581	227	11364
Standard Deviation	5.27	731	19.9	621
CV%	2.3	6.9	8.8	5.5

Recovery

Recovery was not necessary as human FAP shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human FAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

View Larger

Simple Plex Human FAP Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FAP Assay. Assay range is 10.5-40,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fibroblast Activation Protein alpha/FAP

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

Entrez Gene IDs:

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Alternate Names:

170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin