Simple Plex Human FGF-19 Cartridge
R&D Systems | Catalog # SPCKB-PS-000850
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human FGF-19 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 23 |
| Mean (pg/mL) | 96.4 | 4673 | 99.0 | 5398 |
| Standard Deviation | 6.83 | 397 | 8.11 | 628 |
| CV% | 7.1 | 8.5 | 8.2 | 11.6 |
Recovery for Simple Plex Human FGF-19 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=3) | 100 | 97-103 |
Linearity
Samples containing and/or spiked with high concentrations of human FGF-19 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human FGF-19 Cartridge
Human FGF-19 Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: FGF-19
FGF-19 (Fibroblast Growth Factor-19) is a secreted heparin-binding protein in the endocrine subfamily of FGFs that also includes FGF-21 and FGF-23. It is produced by epithelial cells of the ileal intestine in response to bile acids and signals through a receptor complex of FGF R4 and Klotho-beta in the liver. FGF-19 suppresses bile acid synthesis and enhances hepatic protein and glycogen synthesis. Dysregulalted FGF-19 activity is associated with the development of hypercholesterolemia, gall stones, bile acid induced diarrhoea (BAD), hepatocellular cancer, colon cancer, and metabolic syndrome.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional FGF-19 Products
Product Documents for Simple Plex Human FGF-19 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human FGF-19 Cartridge
For research use only
Related Research Areas
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