Simple Plex Human FGF-23 Cartridge
R&D Systems | Catalog # SPCKB-PS-002231
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human FGF-23 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FGF-23 assay.
Inter-Assay Precision (Precision between assays) Replicates of each FGF-23 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FGF-23.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 29 |
| Mean (pg/mL) | 185 | 10,098 | 187 | 10,286 |
| Standard Deviation | 5.43 | 660 | 22.1 | 840 |
| CV% | 2.9 | 6.5 | 11.8 | 8.2 |
Recovery for Simple Plex Human FGF-23 Cartridge
Recovery for the human FGF-23 assays was evaluated at three different spiked concentrations of FGF-23 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 94 | 90-97 |
| Heparin Plasma (n=4) | 99 | 95-102 |
| Serum (n=4) | 102 | 94-108 |
Linearity
Samples containing and/or spiked with high concentrations of human FGF-23 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human FGF-23 Cartridge
Simple Plex Human FGF-23 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FGF-23 Assay. Assay range is 3.78-36,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: FGF-23
FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.
Long Name
Alternate Names
Gene Symbol
Additional FGF-23 Products
Product Documents for Simple Plex Human FGF-23 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human FGF-23 Cartridge
For research use only
Related Research Areas
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