Simple Plex Human Gas6 Cartridge

R&D Systems | Catalog # SPCKB-PS-000491

Simple Plex Human Gas6 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.


Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

1.06 pg/mL

Assay Range

2.62-10000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Gas6 Cartridge Summary

Simple Plex assay is for the detection of human Growth Arrest Specific 6 (Gas6) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Gas6.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Gas6 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Gas6 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Gas6.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 20
Mean (pg/mL) 55.6 2933 53.6 2787
Standard Deviation 3.31 201 3.18 184
CV% 5.9 6.9 5.9 6.6

Recovery for Simple Plex Human Gas6 Cartridge

Recovery was not necessary as human Gas6 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Gas6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Gas6 Assay Linearity

Scientific Data Images for Simple Plex Human Gas6 Cartridge

Simple Plex Human Gas6 Assay Standard Curve

Simple Plex Human Gas6 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Gas6 Assay. Assay range is 2.62-10,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Gas6

Gas6 (Growth Arrest Specific 6) is a secreted protein that contains an extensively gamma-carboxylated N-terminal Gla domain and is dependent upon vitamin K for activity. Gas6 signals through the receptors Axl, Dtk/Tyro3, and Mer and is inhibited by shed soluble forms of Axl and Mer. Gas6 protects cells from stress-induced apoptosis and promotes phagocytosis of apoptotic cell debris, promotes platelet activation but inhibits inflammatory cytokine production, and inhibits VEGF-induced angiogenesis, and regulates tumor cell invasiveness.

Long Name

Growth-arrest-specific Protein 6

Alternate Names

AXLLG

Entrez Gene IDs

2621 (Human); 14456 (Mouse)

Gene Symbol

GAS6

Additional Gas6 Products

Product Documents for Simple Plex Human Gas6 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Gas6 Cartridge

For research use only

Citations for Simple Plex Human Gas6 Cartridge

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