Simple Plex Human GDF-15 Cartridge

R&D Systems | Catalog # SPCKB-PS-000269

Simple Plex Human GDF-15 assay kit contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

0.21 pg/mL

Assay Range

0.52-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human GDF-15 Cartridge Summary

Simple Plex assay for the detection of human Growth Differentiation Factor 15 (GDF-15) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all GDF-15 Simple Plex »

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human GDF-15.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 19 18
Mean (pg/mL) 13.5 571 12.6 599
Standard Deviation 0.898 54.0 1.11 56.0
CV% 6.7 9.5 8.8 9.3

Recovery for Simple Plex Human GDF-15 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 111 110-113

Linearity

Samples containing and/or spiked with high concentrations of human GDF-15 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human GDF-15 Ella Assay Linearity

Scientific Data Images for Simple Plex Human GDF-15 Cartridge

Human GDF-15 Ella Assay Standard Curve

Human GDF-15 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-15

GDF-15 (Growth Differentiation Factor 15) is a dimeric TGF-beta superfamily cytokine that is highly expressed in placenta and brain. It promotes neuronal survival and regulates neuropeptide Y and pro-opiomelanocortin levels to suppress appetite. It is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. GDF-15 also inhibits cartilage formation and the production of TNF-alpha production from LPS-stimulated macrophages.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Entrez Gene IDs

9518 (Human); 23886 (Mouse); 29455 (Rat)

Gene Symbol

GDF15

Additional GDF-15 Products

Product Documents for Simple Plex Human GDF-15 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human GDF-15 Cartridge

For research use only

Citations for Simple Plex Human GDF-15 Cartridge

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