Simple Plex Human Hepcidin Cartridge

Catalog #: SPCKB-PS-000984 Datasheet / COA / SDS
Simple Plex Human Hepcidin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000984
SPCKA-PS-000984
SPCKC-PS-000984
Control Products Available
Human Hepcidin Simple Plex Assay
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Simple Plex Human Hepcidin Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity
4.81 pg/mL
Assay Range
9.7 - 37,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Hepcidin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Hepcidin in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 48 50
Mean (pg/mL) 209 10447 218 12032
Standard Deviation 10.3 789 27.5 1624
CV% 4.9 7.6 12.6 13.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 109 106-112
Heparin Plasma (n=4) 100 92-113
Serum (n=4) 109 106-117

Linearity

Samples containing and/or spiked with high concentrations of human Hepcidin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Hepdicin Simple Plex Assay Linearity

Scientific Data

Human Hepcidiin Simple Plex Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs

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