Simple Plex Human IGFBP-1 Cartridge

R&D Systems | Catalog # SPCKB-PS-000327

Simple Plex Human IGFBP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

0.956 pg/mL

Assay Range

2.63-4020 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human IGFBP-1 Cartridge Summary

Simple Plex assay is for the detection of human Free Insulin-like Growth Factor Binding Protein 1 (Free IGFBP-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IGFBP-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IGFBP-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Free IGFBP-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Free IGFBP-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Free IGFBP-1.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 18 18
Mean (pg/mL) 25.3 1271 27.1 1223
Standard Deviation 1.4 93.9 3.23 65.3
CV% 5.4 7.4 11.9 5.3

Recovery for Simple Plex Human IGFBP-1 Cartridge

Recovery for the human Free IGFBP-1 assays was evaluated at three different spiked concentrations of Free IGFBP-1 protein.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 98 90-111

Linearity

Samples containing and/or spiked with high concentrations of human Free IGFBP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Free IGFBP-1 Assay Linearity

Scientific Data Images for Simple Plex Human IGFBP-1 Cartridge

Simple Plex Human Free IGFBP-1 Assay Standard Curve

Simple Plex Human Free IGFBP-1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Free IGFBP-1 Assay. Assay range is 2.63-4020 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-1

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Long Name

Insulin-like Growth Factor Binding Protein 1

Alternate Names

IGFBP1

Entrez Gene IDs

3484 (Human); 16006 (Mouse)

Gene Symbol

IGFBP1

Additional IGFBP-1 Products

Product Documents for Simple Plex Human IGFBP-1 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human IGFBP-1 Cartridge

For research use only

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