Simple Plex Human IgG Pan Specific Cartridge

R&D Systems | Catalog # SPCKB-PS-007587

Simple Plex Human IgG Pan Specific assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD19, and Wash Buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.


Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

329 pg/mL

Assay Range

371-90560 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human IgG Pan Specific Cartridge Summary

Simple Plex assay is for the detection of human IgG Pan Specific in serum and plasma (EDTA/Heparin) in Sample Diluent SD19.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IgG Pan Specific.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IgG Pan Specific assay.

Inter-Assay Precision (Precision between assays) Replicates of each IgG Pan Specific control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IgG Pan Specific.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 53 54
Mean (pg/mL) 848 40,300 662 37,163
Standard Deviation 41.0 846 82.0 2781
CV% 4.9 2.1 12.3 7.5

Recovery for Simple Plex Human IgG Pan Specific Cartridge

Recovery was not necessary as human IgG Pan Specific shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of IgG Pan Specific were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human IgG Pan Specific Assay Linearity

Scientific Data Images for Simple Plex Human IgG Pan Specific Cartridge

Simple Plex Human IgG Pan Specific Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IgG Pan Specific Assay. Assay range is 371-90,560 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Simple Plex Human IgG Pan Specific Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human IgG Pan Specific Cartridge

For research use only

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