Simple Plex Human IL-12 p70 Cartridge

R&D Systems | Catalog # SPCKB-PS-000498

Simple Plex Human IL-12 p70 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL)

Sensitivity

0.39 pg/mL

Assay Range

0.62-5890 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Simple Plex Human IL-12 p70 Cartridge Summary

Simple Plex assay is for the detection of human Interleukin 12 p70 (IL-12 p70) in cell culture supernatant (CCS), serum, and EDTA plasma in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-12 p70 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-12p70.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-12 p70 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-12 p70 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-12 p70.

Cell Culture Supernates, EDTA Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 15 15
Mean (pg/mL) 36.2 1657 33.2 1587
Standard Deviation 1.25 90.0 1.95 137
CV% 3.4 5.4 5.9 8.6

Recovery for Simple Plex Human IL-12 p70 Cartridge

Recovery for the human IL-12 p70 assays was evaluated at three different spiked concentrations of IL-12 p70 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=6) 87 73-117
Serum (n=6) 81 73-90

Linearity

Samples containing and/or spiked with high concentrations of human IL-12p70 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human IL-12 p70 Assay Linearity

Scientific Data Images for Simple Plex Human IL-12 p70 Cartridge

Simple Plex Human IL-12 p70 Assay Standard Curve

Simple Plex Human IL-12 p70 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-12 p70 Assay. Assay range is 0.62-5890 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts  through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for Simple Plex Human IL-12 p70 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human IL-12 p70 Cartridge

For research use only

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