Simple Plex Human IL-19 Cartridge

Simple Plex Cartridge Kit for quantitation of IL-19. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system
Catalog # Availability Size / Price Qty
SPCKB-PS-002815
SPCKA-PS-002815
SPCKC-PS-002815
Control Products Available
Human IL-19 Simple Plex Assay
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Simple Plex Human IL-19 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Human Milk (3 uL)
Sensitivity
3.07 pg/mL
Assay Range
3.1 - 11,700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)
Specificity
Natural and recombinant human IL-19.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-19 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and human milk

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 55.3 2740 48.9 2355
Standard Deviation 3.1 190 5.07 225
CV% 5.6 6.9 10.4 9.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 96 83-108
Heparin Plasma (n=4) 96 88-106
Serum (n=4) 97 94-99

Linearity

Samples containing and/or spiked with high concentrations of human IL-19 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human IL-19 Simple Plex Assay Linearity

Data Examples

Human IL-19 Simple Plex Assay Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. IL-19 contains seven helices and is secreted as a 35 kDa glycosylated monomer. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24.

Long Name:
Interleukin 19
Entrez Gene IDs:
29949 (Human); 329244 (Mouse)
Alternate Names:
IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19

FAQs

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