Simple Plex Human LBP Cartridge

R&D Systems | Catalog # SPCKB-PS-000508

Simple Plex Human LBP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

6.88 pg/mL

Assay Range

98.3-150000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human LBP Cartridge Summary

Simple Plex assay for the detection of human LBP in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all LBP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human LBP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 37 37
Mean (pg/mL) 1025 51,532 1036 51,807
Standard Deviation 71.4 3306 116 4349
CV% 7.0 6.4 11.1 8.4

Recovery for Simple Plex Human LBP Cartridge

Recovery was not necessary as LBP shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human LBP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human LBP Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human LBP Cartridge

Human LBP Simple Plex Assay Standard Curve

Human LBP Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins that includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipidA portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyzes the binding of LPS monomers to CD14, and promotes LPS-induced immune response.

Long Name

Lipopolysaccharide-binding Protein

Alternate Names

lipopolysaccharide binding protein, lipopolysaccharide-binding protein, LPS-binding protein, MGC22233

Entrez Gene IDs

3929 (Human); 16803 (Mouse)

Gene Symbol

LBP

Additional LBP Products

Product Documents for Simple Plex Human LBP Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human LBP Cartridge

For research use only

Citations for Simple Plex Human LBP Cartridge

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