Simple Plex Human LBP Cartridge

Simple Plex Cartridge Kit for 72 samples, containing LBP for use with Human CCS/Plasma/Serum. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

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Human LBP Simple Plex Assay
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Simple Plex Human LBP Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL)
Sensitivity
98.3 pg/mL
Assay Range
98.3 - 150,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human LBP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human LBP in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assa
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 1025 51532 1036 51807
Standard Deviation 71.4 3306 116 4349
CV% 7 6.4 11.1 8.4

Recovery

Recovery was not necessary as LBP shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human LBP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human LBP Simple Plex Assay Linearity

Data Examples

Human LBP Simple Plex Assay

Human LBP Simple Plex Assay Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins that includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipidA portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyzes the binding of LPS monomers to CD14, and promotes LPS-induced immune response.

Long Name:
Lipopolysaccharide-binding Protein
Entrez Gene IDs:
3929 (Human); 16803 (Mouse)
Alternate Names:
LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233

FAQs

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