Simple Plex Human Lipocalin-2/NGAL Cartridge
Simple Plex Human Lipocalin-2/NGAL Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid
|Intra-Assay Precision||Inter-Assay Precision|
Recovery at three different spiked concentrations within the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=2)||103||102-104|
Human Lipocalin-2/NGAL Ella Assay Standard Curve
Preparation and Storage
The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.
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