Simple Plex Human MAdCAM-1 Cartridge

Catalog #: SPCKB-PS-000510 Datasheet / COA / SDS
Simple Plex Human MAdCAM-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000510
SPCKA-PS-000510
SPCKC-PS-000510
Control Products Available
Human MadCAM-1 Ella Assay
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Simple Plex Human MAdCAM-1 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity
0.65 pg/mL
Assay Range
3.4 - 12,800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human MAdCAM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Mucosal Addressin Cell Adhesion Molecule-1 (MAdCAM-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 53 53
Mean (pg/mL) 129 6645 121 5721
Standard Deviation 11.7 632 13.3 628
CV% 9.1 9.5 11 11

Recovery

Recovery was not necessary as MAdCAM-1 shows natural linearity data from a neat sample to a 1:16 dilution.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 99 87-115

Linearity

Samples containing and/or spiked with high concentrations of human MAdCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human MadCAM-1 Ella Assay Linearity

Scientific Data

Human MadCAM-1 Simple Plex Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyers patches. It has also been found on sinus-lining cells of the spleen.

Long Name:
Mucosal Addressin Cell Adhesion Molecule 1
Entrez Gene IDs:
8174 (Human); 17123 (Mouse)
Alternate Names:
hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1

FAQs

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