Simple Plex Human MAdCAM-1 Cartridge

R&D Systems | Catalog # SPCKB-PS-000510

Simple Plex Human MAdCAM-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

0.65 pg/mL

Assay Range

3.36-12800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human MAdCAM-1 Cartridge Summary

Simple Plex assay for the detection of human Mucosal Addressin Cell Adhesion Molecule-1 (MAdCAM-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human MAdCAM-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 53 53
Mean (pg/mL) 129 6645 121 5721
Standard Deviation 11.7 632 13.3 628
CV% 9.1 9.5 11.0 11.0

Recovery for Simple Plex Human MAdCAM-1 Cartridge

Recovery was not necessary as MAdCAM-1 shows natural linearity data from a neat sample to a 1:16 dilution.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 99 87-115

Linearity

Samples containing and/or spiked with high concentrations of human MAdCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human MadCAM-1 Ella Assay Linearity

Scientific Data Images for Simple Plex Human MAdCAM-1 Cartridge

Human MadCAM-1 Simple Plex Assay Standard Curve

Human MadCAM-1 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyers patches. It has also been found on sinus-lining cells of the spleen.

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Entrez Gene IDs

8174 (Human); 17123 (Mouse)

Gene Symbol

MADCAM1

Additional MAdCAM-1 Products

Product Documents for Simple Plex Human MAdCAM-1 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human MAdCAM-1 Cartridge

For research use only

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