Simple Plex Human Mesothelin Cartridge
R&D Systems | Catalog # SPCKB-PS-000511
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Mesothelin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 22 | 19 |
| Mean (pg/mL) | 299 | 14,366 | 296 | 14,297 |
| Standard Deviation | 10.0 | 615 | 14.5 | 792 |
| CV% | 3.3 | 4.3 | 4.9 | 5.5 |
Recovery for Simple Plex Human Mesothelin Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 105 | 104-105 |
Linearity
Samples containing and/or spiked with high concentrations of human Mesothelin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Mesothelin Cartridge
Human Mesothelin Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Mesothelin
Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.
Alternate Names
Gene Symbol
Additional Mesothelin Products
Product Documents for Simple Plex Human Mesothelin Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human Mesothelin Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human Mesothelin Cartridge
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