Simple Plex Human Mesothelin Cartridge

Catalog #: SPCKB-PS-000511 Datasheet / COA / SDS
Simple Plex Human Mesothelin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000511
SPCKA-PS-000511
SPCKC-PS-000511
Control Products Available
Human Mesothelin Simple Plex Assay
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Simple Plex Human Mesothelin Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity
2.29 pg/mL
Assay Range
13.1 - 50,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Mesothelin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Mesothelin in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 19
Mean (pg/mL) 299 14366 296 14297
Standard Deviation 10 615 14.5 792
CV% 3.3 4.3 4.9 5.5

Recovery

Recovery was not necessary as Mesothelin shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Mesothelin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Mesothelin Ella Assay Linearity

Scientific Data

Human Mesothelin Ella Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.

Entrez Gene IDs:
10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)
Alternate Names:
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

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