Simple Plex Human MICA Cartridge
R&D Systems | Catalog # SPCKB-PS-000197
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human MICA Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 20 | 18 |
| Mean (pg/mL) | 105 | 4434 | 103 | 5230 |
| Standard Deviation | 4.59 | 320 | 8.68 | 533 |
| CV% | 4.4 | 7.2 | 8.4 | 10.2 |
Recovery for Simple Plex Human MICA Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 105 | 101-109 |
| EDTA Plasma (n=4) | 91 | 73-102 |
| Heparin Plasma (n=4) | 96 | 87-102 |
| Serum (n=4) | 99 | 77-111 |
Linearity
Samples containing and/or spiked with high concentrations of human MICA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human MICA Cartridge
Human MICA Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: MICA
MHC class I chain-related genes MICA and MICB are transmembrane glycoproteins that function as ligands for human NKG2D. The two proteins are highly related, sharing 85% amino acid identity, but are also polymorphic. Recognition of MICA or MICB by NKG2D results in the activation of NK cell cytolytic activity and/or cytokine production. MICA/B are minimally expressed on normal cells, but are frequently expressed on or shed from epithelial tumors and can be induced by bacterial and viral infections. MICA and MICB recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional MICA Products
Product Documents for Simple Plex Human MICA Cartridge
Product Specific Notices for Simple Plex Human MICA Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human MICA Cartridge
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