Simple Plex Human MMP-9 Cartridge

R&D Systems | Catalog # SPCKB-PS-000661

Simple Plex Human MMP-9 assay kit contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL)

Sensitivity

10.5 pg/mL

Assay Range

40-152700 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Simple Plex Human MMP-9 Cartridge Summary

Simple Plex assay is for the detection of human Matrix Metalloproteinase 9 (MMP-9) in cell culture supernatant (CCS), serum, and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all MMP-9 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human MMP-9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) ach control was tested multiple times in one MMP-9 assay.

Inter-Assay Precision (Precision between assays) Replicates of each MMP-9 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MMP-9.

Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 24
Mean (pg/mL) 979 50,388 974 48,013
Standard Deviation 48.2 3412 84.4 4337
CV% 4.9 6.8 8.7 9.0

Recovery for Simple Plex Human MMP-9 Cartridge

Recovery for the human MMP-9 assays was evaluated at three different spiked concentrations of MMP-9 protein.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=2) 100 96-104

Linearity

Samples containing and/or spiked with high concentrations of human MMP-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human MMP-9 Assay Linearity

Scientific Data Images for Simple Plex Human MMP-9 Cartridge

Simple Plex Human MMP-9 Assay Standard Curve

Simple Plex Human MMP-9 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human MMP-9 Assay. Assay range is 40.0-152,700 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

MMP-9 (Gelatinase B) is a metalloproteinase that degrades multiple extracellular matrix components including Gelatin, Collagens III, IV, V, and XI, Elastin, Nidogen-1, and Vitronectin. It also cleaves a variety of chemokines and growth factors (e.g. IL-1 beta, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta, membrane bound TNF-alpha, VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein. MMP-9 can trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and CD25/IL-2 R alpha). The activity of MMP-9 is regulated by its association with the TIMP-1 protease inhibitor. Circulating levels of MMP-9 are elevated in many inflammatory disorders including intraluminal thrombus formation, atherosclerosis, Crohn’s disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for Simple Plex Human MMP-9 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human MMP-9 Cartridge

For research use only

Citations for Simple Plex Human MMP-9 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human MMP-9 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human MMP-9 Cartridge and earn rewards!

Have you used Simple Plex Human MMP-9 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card