Home / Pentraxin 3/TSG-14 / Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Catalog #: SPCKB-PS-000537 Datasheet / COA / SDS
Simple Plex Human Pentraxin 3/TSG-14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000537
SPCKA-PS-000537
SPCKC-PS-000537
Control Products Available
898213: Simple Plex Control for Human Pentraxin 3/TSG-14
Human Pentraxin 3/TSG-14 Ella Assay
3 Images
Product Details
FAQs
Supplemental Products
Reviews
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Pentraxin 3/TSG-14 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Saliva (5 uL)
Sensitivity
5.2 pg/mL
Assay Range
5.2 - 32,530 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human Pentraxin 3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Pentraxin 3 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and saliva.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 17
Mean (pg/mL) 206 9568 211 9837
Standard Deviation 10.1 46 11.7 552
CV% 4.9 4.8 5.5 5.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 105 103-110
EDTA Plasma (n=4) 98 92-109
Heparin Plasma (n=4) 102 98-109

Linearity

Samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Pentraxin 3/TSG-14 Ella Assay Linearity

Scientific Data

N/A Human Pentraxin 3/TSG-14 Ella Assay Standard Curve View Larger

Human Pentraxin 3/TSG-14 Ella Assay Standard Curve

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name:
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs:
5806 (Human); 19288 (Mouse)
Alternate Names:
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs

Automated ELISA Instruments

Ella Automated Immunoassay System

600-100
1 Review
3 Images
ELISA

Simple Plex Assay Controls

Simple Plex Control for Human Pentraxin 3/TSG-14

898213
Ctrl

Reviews for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Pentraxin 3/TSG-14 Cartridge and earn rewards!

Have you used Simple Plex Human Pentraxin 3/TSG-14 Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review