Simple Plex Human Pentraxin 3/TSG-14 Cartridge

R&D Systems | Catalog # SPCKB-PS-000537

Simple Plex Human Pentraxin 3/TSG-14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL)

Sensitivity

2.26 pg/mL

Assay Range

5.2-32530 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Simple Plex Human Pentraxin 3/TSG-14 Cartridge Summary

Simple Plex assay for the detection of human Pentraxin 3 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and saliva.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Pentraxin 3/TSG-14 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Pentraxin 3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 17
Mean (pg/mL) 206 9568 211 9837
Standard Deviation 10.1 46 11.7 552
CV% 4.9 4.8 5.5 5.6

Recovery for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 105 103-110
EDTA Plasma (n=4) 98 92-109
Heparin Plasma (n=4) 102 98-109

Linearity

Samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Pentraxin 3/TSG-14 Ella Assay Linearity

Scientific Data Images for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Human Pentraxin 3/TSG-14 Ella Assay Standard Curve

Human Pentraxin 3/TSG-14 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name

Tumor Necrosis Factor Stimulated Gene 14

Alternate Names

PTX3, TSG-14, TSG14

Entrez Gene IDs

5806 (Human); 19288 (Mouse)

Gene Symbol

PTX3

Additional Pentraxin 3/TSG-14 Products

Product Documents for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

For research use only

Citations for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human Pentraxin 3/TSG-14 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Pentraxin 3/TSG-14 Cartridge and earn rewards!

Have you used Simple Plex Human Pentraxin 3/TSG-14 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card