Simple Plex Human Pro-GRP Cartridge

R&D Systems | Catalog # SPCKB-PS-000922

Simple Plex Human Pro-GRP/Gastrin-releasing Peptide/Bombesin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.53 pg/mL

Assay Range

2.62-4000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Pro-GRP Cartridge Summary

Simple Plex assay is for the detection of human Pro-Gastrin-releasing Peptide (Pro-GRP) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Gastrin-releasing Peptide/Bombesin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Pro-GRP.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Pro-GRP assay.

Inter-Assay Precision (Precision between assays) Replicates of each Pro-GRP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Pro-GRP.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 5 5
Mean (pg/mL) 27.2 1566 27.1 1561
Standard Deviation 2.18 105 2.94 129
CV% 8.0 6.7 10.9 8.2

Recovery for Simple Plex Human Pro-GRP Cartridge

Recovery for the human Pro-GRP assays was evaluated at three different spiked concentrations of Pro-GRP protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=3) 92 88-99
Heparin Plasma (n=3) 94 90-96
Serum (n=3) 101 93-107

Linearity

Samples containing and/or spiked with high concentrations of human Pro-GRP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Pro-GRP Assay Linearity

Scientific Data Images for Simple Plex Human Pro-GRP Cartridge

Simple Plex Human Pro-GRP Assay Standard Curve

Simple Plex Human Pro-GRP Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Pro-GRP Assay. Assay range is 2.62-4000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Gastrin-releasing Peptide/Bombesin

Gastrin-releasing peptide receptor (GRPR) is a Bombesin Receptor. In addition to its involvement in autism and mental retardation, GRPR may also play a role in the progression of cancer. Nicotine exposure induces an increase in GRPR expression in the lung Shriver et al. (2000). GRPR has been reported in brain, breast, colon, lung, lymph node, ovary, placenta, prostate, stomach, and uterus. It has also been shown to be expressed in several cancers, including those in breast, colon, lung, ovary, pancreas, and prostate.

Alternate Names

Bombesin, Gastrinreleasing Peptide, GRP, GRP10

Entrez Gene IDs

2922 (Human); 225642 (Mouse); 171101 (Rat)

Gene Symbol

GRP

Additional Gastrin-releasing Peptide/Bombesin Products

Product Documents for Simple Plex Human Pro-GRP Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Pro-GRP Cartridge

For research use only

Citations for Simple Plex Human Pro-GRP Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human Pro-GRP Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Pro-GRP Cartridge and earn rewards!

Have you used Simple Plex Human Pro-GRP Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card