Simple Plex Human Procalcitonin Cartridge

R&D Systems | Catalog # SPCKB-PS-001144

Simple Plex Human Procalcitonin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

0.79 pg/mL

Assay Range

1.58-15100 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Simple Plex Human Procalcitonin Cartridge Summary

Simple Plex assay for the detection of human Procalcitonin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Procalcitonin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Procalcitonin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 18 17
Mean (pg/mL) 81.2 4828 83.3 4876
Standard Deviation 5.15 301 4.69 321
CV% 6.3 6.2 5.6 6.6

Recovery for Simple Plex Human Procalcitonin Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=3) 109 106-113
EDTA Plasma (n=4) 105 97-111
Heparin Plasma (n=4) 103 98-111
Serum (n=4) 105 96-110

Linearity

Samples containing and/or spiked with high concentrations of human Procalcitonin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Procalcitonin Ella Assay Linearity

Scientific Data Images for Simple Plex Human Procalcitonin Cartridge

Human Procalcitonin Ella Assay Standard Curve

Human Procalcitonin Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Procalcitonin

Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.

Alternate Names

CALC1, CALCA, Calcitonin 1, calcitonin gene-related peptide 1, CGRP, Katacalcin

Entrez Gene IDs

796 (Human); 12310 (Mouse); 24241 (Rat)

Gene Symbol

CALCA

Additional Procalcitonin Products

Product Documents for Simple Plex Human Procalcitonin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Procalcitonin Cartridge

For research use only

Citations for Simple Plex Human Procalcitonin Cartridge

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