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Simple Plex Human RAGE Cartridge

R&D Systems | Catalog # SPCKB-PS-000341

Simple Plex Human RAGE/AGER assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.82 pg/mL

Assay Range

12.4-7570 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human RAGE Cartridge Summary

Simple Plex assay for the detection of human Receptor for Advanced Glycation End product (RAGE) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all RAGE/AGER Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human RAGE.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 19 19
Mean (pg/mL) 49.6 2301 45.3 2263
Standard Deviation 3.15 175 3.16 247
CV% 6.4 7.6 7.0 10.9

Recovery for Simple Plex Human RAGE Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 108 106-110

Linearity

Samples containing and/or spiked with high concentrations of human RAGE were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Human RAGE Ella Assay Linearity

Scientific Data Images for Simple Plex Human RAGE Cartridge

Human RAGE Ella Assay Standard Curve

Human RAGE Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RAGE/AGER

RAGE (Receptor for Advanced Glycation End product) is a transmembrane glycoprotein that binds advanced glycation end products (AGEs), beta-amyloid peptides, HMGB1/Amphoterin, and several S100 family proteins. AGEs are adducts formed by the non-enzymatic glycation and oxidation of proteins and lipids. A soluble form can also be generated by MMP-mediated shedding. RAGE is expressed in the CNS during development as well as in adult endothelial cells, smooth muscle cells, pericytes, monocytes, and neurons. It is locally upregulated in vascular inflammation (e.g. diabetes, atherosclerosis, vascular injury, Alzheimer’s disease). At these sites, RAGE binding to S100A1, EN-RAGE/S100A12, or S100B induces inflammatory immune cell adhesion and infiltration as well as vascular smooth muscle proliferation, neointimal expansion, atherosclerotic plaque development, and transport of A-beta into the cerebrospinal fluid. In cancer, RAGE binding to HMGB1, S100A8, or S100A9 promotes tumor growth and metastasis in addition to inflammatory cell infiltration.

Long Name

Receptor for Advanced Glycation End Products

Alternate Names

AGER, SCARJ1

Entrez Gene IDs

177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)

Gene Symbol

AGER

Additional RAGE/AGER Products

Product Documents for Simple Plex Human RAGE Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human RAGE Cartridge

For research use only

Citations for Simple Plex Human RAGE Cartridge

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