Simple Plex Human S100B Cartridge

Catalog #: SPCKC-PS-009486 Datasheet / COA / SDS

Simple Plex Human S100B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)

Catalog #	Availability	Size / Price	Qty
SPCKC-PS-009486
SPCKA-PS-009486
ST01C-PS-009486

Control Products Available

898575: Simple Plex Control for Human/Mouse/Rat S100B

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human S100B Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

0.648 pg/mL

Assay Range

4.0 - 2,448 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid)

Specificity

Natural and recombinant S100B.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human S100 Calcium Binding Protein B (S100B) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	72	71
Mean (pg/mL)	32	1845	23.5	1443
Standard Deviation	2.09	67.3	2.16	115
CV%	6.6	3.6	9.2	8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	93-108
EDTA Plasma (n=4)	96	92-99
Heparin Plasma (n=4)	94	90-98
Saliva (n=3)	89	82-94
Serum (n=4)	94	90-101

Linearity

Samples containing and/or spiked with high concentrations of S100B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human S100B Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha-Synuclein

The synuclein family includes alpha-Synuclein, beta-Synuclein, gamma-Synuclein, and synoretin. These small, highly conserved proteins are localized predominantly in the brain, concentrated in presynaptic nerve terminals. Alpha-Synuclein is normally a soluble protein. Abnormal alpha-synuclein forms filamentous inclusions (Lewy bodies and Lewy neurites) in several neurodegenerative diseases, including Parkinson's disease (PD), dementia with Lewy bodies, and Alzheimer's disease. Beta-Synuclein and gamma-Synuclein (synoretin) associate with alpha-synuclein and inhibit its aggregation.

Entrez Gene IDs:

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names:

alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein