Simple Plex Human Siglec-9 Cartridge

R&D Systems | Catalog # SPCKB-PS-003159

Simple Plex Human Siglec-9 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.46 pg/mL

Assay Range

1.57-15000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Siglec-9 Cartridge Summary

Simple Plex assay is for the detection of human Sialic acid-binding Ig-like lectin-9 (Siglec-9) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Siglec-9 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Siglec-9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Siglec-9 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Siglec-9 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Siglec-9.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 44 44
Mean (pg/mL) 98.3 4615 94.1 4531
Standard Deviation 3.60 366 5.85 377
CV% 3.7 7.9 6.2 8.3

Recovery for Simple Plex Human Siglec-9 Cartridge

Recovery was not necessary as human Siglec-9 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Siglec-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Siglec-9 Assay Linearity

Scientific Data Images for Simple Plex Human Siglec-9 Cartridge

Simple Plex Human Siglec-9 Assay Standard Curve

Simple Plex Human Siglec-9 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Siglec-9 Assay. Assay range is 1.57-15,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Siglec-9

Siglecs are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal, Ig-like V-type domain that mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (2 to 17), a single transmembrane region, and a cytoplasmic tail. The siglecs can be broadly classified into two subgroups: Siglecs-1, -2, and -4, and a Siglec-3/CD33-related subgroup (Siglecs-3, and -5 through -13 in primates) defined by sequence similarity and clustered gene localization. They are widely expressed on hematopoietic cells, often in a cell-type-specific manner, and Siglec-4/MAG is a myelin component in Schwann cells and oligodendrocytes. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Although Siglec functions continue to be defined, most have intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIM), implicating them in the suppression of immunoreceptor signaling. They may also participate in cell/cell interactions or act as receptors for the entry of viral or bacterial pathogens.

Long Name

Sialic Acid Binding Ig-like Lectin 9

Alternate Names

CD329, OBBP-Like, Siglec9

Entrez Gene IDs

27180 (Human); 102118310 (Cynomolgus Monkey)

Gene Symbol

SIGLEC9

Additional Siglec-9 Products

Product Documents for Simple Plex Human Siglec-9 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Siglec-9 Cartridge

For research use only

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