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Simple Plex Human SP-D Cartridge

Catalog #: SPCKB-PS-000529 Datasheet / COA / SDS
Simple Plex Human SP-D assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000529
SPCKA-PS-000529
SPCKC-PS-000529
Control Products Available
898225: Simple Plex Control for Human SP-D
Human SP-D Simple Plex Assay
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human SP-D Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
19.1 pg/mL
Assay Range
154.0 - 93,810 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human SP-D.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human SP-D in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 25
Mean (pg/mL) 628 26121 532 26305
Standard Deviation 48.7 1603 61.3 3115
CV% 7.8 6.1 11.5 11.8

Recovery

Recovery was not necessary as SP-D shows natural linearity data from a neat sample to a 1:16 dilution.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 105 94-125

Linearity

Samples containing and/or spiked with high concentrations of human SP-D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human SP-D Ella Assay Linearity

Scientific Data

N/A Human SP-D Simple Plex Assay Standard Curve View Larger

Human SP-D Ella Assay Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Long Name:
Surfactant Pulmonary Associated Protein D
Entrez Gene IDs:
6441 (Human); 20390 (Mouse)
Alternate Names:
COLEC7collectin-7; Collectin 7; Collectin-7; Lung surfactant protein D; PSPD; PSP-D; SFTP4; SFTP4pulmonary surfactant-associated protein D; SFTPD; SPD; SP-D; SP-Dpulmonary surfactant apoprotein; surfactant protein D; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4

FAQs

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