Simple Plex Human Thrombospondin-1 Cartridge
R&D Systems | Catalog # SPCKB-PS-000531
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Thrombospondin-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Thrombospondin-1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Thrombospondin-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Thrombospondin-1.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 19 | 19 |
| Mean (pg/mL) | 1815 | 95,787 | 1904 | 92,902 |
| Standard Deviation | 163 | 8339 | 264 | 8120 |
| CV% | 9.0 | 8.7 | 13.9 | 8.7 |
Recovery for Simple Plex Human Thrombospondin-1 Cartridge
Recovery for the human Thrombospondin-1 assays was evaluated at three different spiked concentrations of Thrombospondin-1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Platelet-poor EDTA Plasma (n=6) | 101 | 94-104 |
Linearity
Samples containing and/or spiked with high concentrations of human Thrombospondin-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Thrombospondin-1 Cartridge
Simple Plex Human Thrombospondin-1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Thrombospondin-1 Assay. Assay range is 498-303,880 pg/mLPreparation and Storage
Shipping
Stability & Storage
Background: Thrombospondin-1
Thrombospondin-1 (TSP-1) is a secreted homotrimeric protein that regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 counteracts the angiogenic, hypotensive, and anti-thrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP). TSP-1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling. In the nervous system, it promotes excitatory synapse formation and supports the integration of neural progenitor cells into the olfactory bulb.
Alternate Names
Gene Symbol
Additional Thrombospondin-1 Products
Product Documents for Simple Plex Human Thrombospondin-1 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human Thrombospondin-1 Cartridge
For research use only
Related Research Areas
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