Simple Plex Human Tie-2 Cartridge
R&D Systems | Catalog # SPCKB-PS-000532
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Tie-2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 7 | 8 |
| Mean (pg/mL) | 222 | 13660 | 253 | 12254 |
| Standard Deviation | 15.7 | 831 | 33.4 | 960 |
| CV% | 7.1 | 6.1 | 13.2 | 7.8 |
Recovery for Simple Plex Human Tie-2 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=3) | 115 | 113-118 |
Linearity
Samples containing and/or spiked with high concentrations of human Tie-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Tie-2 Cartridge
Human Tie-2 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Tie-2
Tie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult endothelial cells, hematopoietic stem cells, and a circulating population of proangiogenic monocytes. Tie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular integrity by mediating endothelial cell-smooth muscle cell communication and inhibiting endothelial cell apoptosis. It is also required for embryonic development of the endocardium. In addition, Tie-2 signaling mediates the quiescence of bone marrow stem cells in response to osteoblast-produced Angiopoietin-1. This quiescence is critical for maintaining an ongoing hematopoietic capability in the bone marrow.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional Tie-2 Products
Product Documents for Simple Plex Human Tie-2 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human Tie-2 Cartridge
For research use only
Citations for Simple Plex Human Tie-2 Cartridge
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
