Simple Plex Human TIMP-1 Cartridge

Catalog #: SPCKB-PS-001442 Datasheet / COA / SDS

Simple Plex Human TIMP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001442
SPCKA-PS-001442
SPCKC-PS-001442

Control Products Available

898104: Simple Plex Control for Human TIMP-1

Ella Automated ELISA And Simple Plex Human TIMP-1 Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human TIMP-1 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

0.34 pg/mL

Assay Range

1.1 - 10,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Specificity

Natural and recombinant human TIMP-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex™ assay is for the detection of human Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TIMP-1 assay

Inter-Assay Precision (Precision between assays) Replicates of each TIMP-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TIMP-1

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	33
Mean (pg/mL)	57.8	2915	54.5	2833
Standard Deviation	4.86	152	5.97	259
CV%	8.4	5.2	10.9	9.1

Recovery

Recovery for the human TIMP-1 assays was evaluated at three different spiked concentrations of TIMP-1 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	112	112-113

Linearity

Samples containing and/or spiked with high concentrations of TIMP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

Simple Plex Human TIMP-1 Assay Standard Curve

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Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TIMP-1 Assay. Assay range is 1.05-10,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-1

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name:

Tissue Inhibitors of Metalloproteinases 1

Entrez Gene IDs:

7076 (Human); 21857 (Mouse); 116510 (Rat)

Alternate Names:

CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1