Simple Plex Human Total MMP-8 Cartridge

R&D Systems | Catalog # SPCKB-PS-009199

Simple Plex Human Total MMP-8 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (25 µL), Platelet-poor EDTA Plasma (25 µL), Heparin Plasma (25 µL), Platelet-poor Heparin Plasma (25 µL), Saliva (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

1.1 pg/mL

Assay Range

4.99-19007 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Cerebrospinal Fluid)

Simple Plex Human Total MMP-8 Cartridge Summary

Simple Plex assay for the detection of human Total MMP-8 (pro- and active MMP-8) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), platelet-poor plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all MMP-8 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural human active, pro, and TIMP-1 complexed MMP-8. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 48 48
Mean (pg/mL) 119 5958 140 6325
Standard Deviation 4.09 205 14.8 496
CV% 3.4 3.4 10.6 7.8

Recovery for Simple Plex Human Total MMP-8 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 100 95-106
Cerebrospinal Fluid (n=3) 94 89-97

Linearity

Samples containing and/or spiked with high concentrations of human Total MMP-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human Total MMP-8 Cartridge

Human Total MMP-8 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-8

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name

Matrix Metalloproteinase 8

Alternate Names

Collagenase 2, MMP8

Entrez Gene IDs

4317 (Human); 17394 (Mouse); 63849 (Rat)

Gene Symbol

MMP8

Additional MMP-8 Products

Product Documents for Simple Plex Human Total MMP-8 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Total MMP-8 Cartridge

For research use only

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