Simple Plex Human Trappin-2/Elafin Cartridge

R&D Systems | Catalog # SPCKB-PS-000228

Simple Plex Human Trappin-2/Elafin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.12 pg/mL

Assay Range

3.78-14400 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Trappin-2/Elafin Cartridge Summary

Simple Plex assay is for the detection of human Trappin serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Trappin-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Trappin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Trappin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Trappin.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 18 18
Mean (pg/mL) 92.0 4838 102 4665
Standard Deviation 8.1 427 13.0 339
CV% 8.8 8.8 12.8 7.3

Recovery for Simple Plex Human Trappin-2/Elafin Cartridge

Recovery was not necessary as human Trappin shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Trappin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Trappin Assay Linearity

Scientific Data Images for Simple Plex Human Trappin-2/Elafin Cartridge

Simple Plex Human Trappin Assay Standard Curve

Simple Plex Human Trappin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Trappin Assay. Assay range is 3.78-14,400 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Trappin-2/Elafin

Trappin-2 is a member of the trappin family that includes the SLPI (secretory leukocyte protease inhibitor) protein. Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leukoproteinase) are alternative names for trappin-2 and reflect its protease targets. However, Elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule.

Long Name

Elastase Inhibitor, Transglutaminase Substrate, Serine Protease

Alternate Names

Elafin, ESI, PI3, SKALP, Trappin2

Entrez Gene IDs

5266 (Human)

Gene Symbol

PI3

Additional Trappin-2/Elafin Products

Product Documents for Simple Plex Human Trappin-2/Elafin Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Trappin-2/Elafin Cartridge

For research use only

Related Research Areas

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