Simple Plex Human TREM-1 Cartridge

Catalog #: SPCKB-PS-001020 Datasheet / COA / SDS
Simple Plex Human TREM-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-001020
SPCKA-PS-001020
SPCKC-PS-001020
Control Products Available
Human TREM-1 Ella Assay
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Simple Plex Human TREM-1 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL)
Sensitivity
4.19 pg/mL
Assay Range
4.2 - 40,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human TREM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Triggering Receptor Expressed on Myeloid cells (TREM-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 24 23
Mean (pg/mL) 216 11034 220 11170
Standard Deviation 17.9 1013 20.1 673
CV% 8.3 9.2 9.1 6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=3) 119 115-123
Urine (n=5) 118 113-125

Linearity

Samples containing and/or spiked with high concentrations of human TREM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human TREM-1 Ella Assay Linearity

Scientific Data

Human TREM-1 Ella Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TREM-1

The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.

Long Name:
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs:
54210 (Human); 58217 (Mouse)
Alternate Names:
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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