Simple Plex Human uPAR Cartridge

Catalog #: SPCKB-PS-007370 Datasheet / COA / SDS
Simple Plex Human uPAR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer
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Human uPAR Simple Plex Assay
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Simple Plex Human uPAR Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (5 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (5 uL), Urine (5 uL), Cerebrospinal Fluid (10 uL)
1.68 pg/mL
Assay Range
1.7 - 160,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)
Natural and recombinant human uPAR.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Summary

Simple Plex assay for the detection of human Urokinase-type Plasminogen Activator Receptor (uPAR) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, urine and cerebrospinal fluid (CSF)


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 120 6099 110 5748
Standard Deviation 6.8 261 8.51 558
CV% 5.7 4.3 7.7 9.7


Recovery was not necessary at uPAR shows natural linearity data from a neat sample to a 1:16 dilution. 


Samples containing and/or spiked with high concentrations of uPAR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human uPAR Simple Plex Assay

Scientific Data

Human uPAR Simple Plex Assay Standard Curve

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: uPAR

The urokinase-type Plasminogen Activator Receptor (uPAR) is a GPI-anchored protein that binds to both the inactive and active forms of uPA. This interaction serves to localize and enhance the proteolytic activity of uPA, leading to increased conversion of Plasminogen to Plasmin. uPA also triggers uPAR-mediated signal transduction, activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can additionally regulate cell adhesion by direct binding to Vitronectin and preventing Integrins from binding to their ligands.

Long Name:
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs:
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names:
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3


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