Simple Plex Human uPAR Cartridge

R&D Systems | Catalog # SPCKB-PS-007370

Simple Plex Human uPAR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (10 µL), Urine (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

0.2 pg/mL

Assay Range

1.68-16000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)

Simple Plex Human uPAR Cartridge Summary

Simple Plex assay is for the detection of human Urokinase-type Plasminogen Activator Receptor (uPAR) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, urine, and cerebrospinal fluid (CSF) in Sample Diluent SD10.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all uPAR Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human uPAR.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one uPAR assay.

Inter-Assay Precision (Precision between assays) Replicates of each uPAR control were tested in multiple assays performed by at least three technicians using two lots of reagents for human uPAR.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 49 50
Mean (pg/mL) 120 6099 110 5748
Standard Deviation 6.80 261 8.51 558
CV% 5.7 4.3 7.7 9.7

Recovery for Simple Plex Human uPAR Cartridge

Recovery was not necessary as human uPAR shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of uPAR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human uPAR Assay Linearity

Scientific Data Images for Simple Plex Human uPAR Cartridge

Simple Plex Human uPAR Assay Standard Curve

Simple Plex Human uPAR Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human uPAR Assay. Assay range is 1.68-16,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: uPAR

The urokinase-type Plasminogen Activator Receptor (uPAR) is a GPI-anchored protein that binds to both the inactive and active forms of uPA. This interaction serves to localize and enhance the proteolytic activity of uPA, leading to increased conversion of Plasminogen to Plasmin. uPA also triggers uPAR-mediated signal transduction, activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can additionally regulate cell adhesion by direct binding to Vitronectin and preventing Integrins from binding to their ligands.

Long Name

Urokinase-type Plasminogen Activator Receptor

Alternate Names

PLAUR

Entrez Gene IDs

5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)

Gene Symbol

PLAUR

Additional uPAR Products

Product Documents for Simple Plex Human uPAR Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human uPAR Cartridge

For research use only

Citations for Simple Plex Human uPAR Cartridge

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