Simple Plex Human VEGF-B Cartridge

Simple Plex Cartridge Kit for 72 samples, containing VEGF-B for use with Human Plasma/Serum. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

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Simple Plex Human VEGF-B Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
8.83 pg/mL
Assay Range
8.8 - 15,830 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human VEGF-B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human VEGF-B in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assa
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 208 10568 206 10533
Standard Deviation 10 759 17.6 1312
CV% 4.8 7.2 8.5 12.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 101 95-109
Heparin Plasma (n=4) 98 90-104
Serum (n=4) 100 95-103

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity.

Long Name:
Vascular Endothelial Growth Factor B
Entrez Gene IDs:
7423 (Human); 22339 (Mouse)
Alternate Names:
vascular endothelial growth factor B; VEGFB; VEGF-B; VEGF-related factor; VRFVEGFL

FAQs

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