Simple Plex Human VEGF-B Cartridge

R&D Systems | Catalog # SPCKB-PS-000539

Simple Plex Human VEGF-B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.


Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

4.49 pg/mL

Assay Range

10.08-15384 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human VEGF-B Cartridge Summary

Simple Plex assay is for the detection of human Vascular Endothelial Growth Factor B (VEGF-B) in serum and plasma (EDTA/heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human VEGF-B.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one VEGF-B assay.

Inter-Assay Precision (Precision between assays) Replicates of each VEGF-B control were tested in multiple assays performed by at least three technicians using two lots of reagents for human VEGF-B.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 21
Mean (pg/mL) 208 10,568 206 10,533
Standard Deviation 10.0 759 17.6 1312
CV% 4.8 7.2 8.5 12.5

Recovery for Simple Plex Human VEGF-B Cartridge

Recovery for the human VEGF-B assays was evaluated at three different spiked concentrations of VEGF-B protein..

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 101 95-109
Heparin Plasma (n=4) 98 90-104
Serum (n=4) 100 95-103

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human VEGF-B Assay Linearity

Scientific Data Images for Simple Plex Human VEGF-B Cartridge

Simple Plex Human VEGF-B Assay Standard Curve

Simple Plex Human VEGF-B Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human VEGF-B Assay. Assay range is 2.62-4000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity.

Long Name

Vascular Endothelial Growth Factor B

Alternate Names

VEGFB

Entrez Gene IDs

7423 (Human); 22339 (Mouse)

Gene Symbol

VEGFB

Additional VEGF-B Products

Product Documents for Simple Plex Human VEGF-B Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human VEGF-B Cartridge

For research use only

Citations for Simple Plex Human VEGF-B Cartridge

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