Simple Plex Human VEGF-C Cartridge

R&D Systems | Catalog # SPCKB-PS-000348

Simple Plex Human VEGF-C assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), Platelet-poor EDTA Plasma (25 µL), Platelet-poor Heparin Plasma (25 µL)

Sensitivity

6.9 pg/mL

Assay Range

32.6-49690 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Simple Plex Human VEGF-C Cartridge Summary

Simple Plex assay is for the detection of human Vascular Endothelial Growth Factor C (VEGF-C) in serum and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD10.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all VEGF-C Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Species

Human

Specificity

Natural and recombinant human VEGF-C.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one VEGF-C assay.

Inter-Assay Precision (Precision between assays) Replicates of each VEGF-C control were tested in multiple assays performed by at least three technicians using two lots of reagents for human VEGF-C.

Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 19 19
Mean (pg/mL) 149 7683 151 7918
Standard Deviation 13.7 531 13.4 727
CV% 9.2 6.9 8.8 9.2

Recovery for Simple Plex Human VEGF-C Cartridge

Recovery for the human VEGF-C assays was evaluated at three different spiked concentrations of VEGF-C protein.

Sample Type Average % Recovery Range %
Platelet-poor EDTA Plasma (n=4) 111 92-122
Platelet-poor Heparin Plasma (n=4) 111 90-123

Linearity

Samples containing and/or spiked with high concentra-tions of human VEGF-C were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human VEGF-C Assay Linearity

Scientific Data Images for Simple Plex Human VEGF-C Cartridge

Simple Plex Human VEGF-C Assay Standard Curve

Simple Plex Human VEGF-C Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human VEGF-C Assay. Assay range is 32.6-49,690 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Entrez Gene IDs

7424 (Human); 22341 (Mouse)

Gene Symbol

VEGFC

Additional VEGF-C Products

Product Documents for Simple Plex Human VEGF-C Cartridge

Certificate of Analysis

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Product Specific Notices for Simple Plex Human VEGF-C Cartridge

For research use only

Citations for Simple Plex Human VEGF-C Cartridge

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