Simple Plex Mouse Cytokine Panel

R&D Systems | Catalog # ST01C-MP-004435

Simple Plex 32x4 mouse panel containing IL-2, IL-6, IFN-gamma, and TNF-alpha with sample diluent SD09. Sample type indicated with MP (Plasma/Serum).
R&D Systems
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Key Product Details

Features

The Simple Plex Mouse Cytokine Panel is for the detection of mouse IL-2, IL-6, IFNg, and TNFa in plasma/serum samples.

 
Sample Type & Volume Required Per Well Plasma/Serum (25 uL)
Sensitivity IL-2 0.45 pg/mL; IL-6 0.15 pg/mL; IFNg 0.48 pg/mL; TNFa 0.06 pg/mL
Assay Range IL-2 3-1827 pg/mL; IL-6 0.6-5770 pg/mL; IFNg 0.94-9000 pg/mL; TNFa 0.31-2930 pg/mL

Species

Mouse

Applications

Simple Plex Automated Immunoassay

Product Summary for Simple Plex Mouse Cytokine Panel

Product Specifications

Reactivity Mouse
Specificity Natural and recombinant mouse IL-2, IL-6, IFNg, and TNFa.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IL-2 n 16 16 16 16
Mean (pg/mL) 12.6 496 12.8 616
Standard Deviation 0.736 37 1.61 67.5
CV (%) 5.8 7.5 12.6 11.0
IL-6 n 16 16 27 27
Mean (pg/mL) 31.3 1766 33 1722
Standard Deviation 2.67 177 2.78 143
CV (%) 8.5 10 8.4 8.3
IFNg n 16 16 51 51
Mean (pg/mL) 44.4 2296 51.7 2486
Standard Deviation 1.33 205 5.35 263
CV (%) 3.0 8.9 10.4 10.6
TNFa n 16 16 32 32
Mean (pg/mL) 8.38 391 8.30 427
Standard Deviation 0.748 38.1 1.08 53.4
CV (%) 8.9 9.8 13.0 12.5
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Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Simple Plex Mouse Cytokine Panel

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse Cytokine Panel

For research use only

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