Simple Plex Mouse IL-12 p70 Cartridge
R&D Systems | Catalog # SPCKB-MP-009486
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse IL-12 p70 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 51 | 53 |
| Mean (pg/mL) | 24.0 | 1105 | 23.9 | 1088 |
| Standard Deviation | 0.67 | 28.0 | 2.52 | 77.9 |
| CV% | 2.8 | 2.5 | 10.6 | 7.2 |
Recovery for Simple Plex Mouse IL-12 p70 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 104 | 102-106 |
| EDTA Plasma (n=4) | 111 | 109-114 |
| Heparin Plasma (n=4) | 96 | 91-98 |
| Serum (n=4) | 106 | 103-108 |
| Urine (n=4) | 90 | 82-93 |
Linearity
Samples containing and/or spiked with high concentrations of mouse IL-12 p70 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse IL-12 p70 Cartridge
Mouse IL-12 p70 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-12 p70
IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.
Long Name
Alternate Names
Gene Symbol
Additional IL-12 p70 Products
Product Documents for Simple Plex Mouse IL-12 p70 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Mouse IL-12 p70 Cartridge
For research use only
Citations for Simple Plex Mouse IL-12 p70 Cartridge
View all citations for the Simple Plex platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
Powered by Bioz
Customer Reviews for Simple Plex Mouse IL-12 p70 Cartridge
There are currently no reviews for this product. Be the first to review Simple Plex Mouse IL-12 p70 Cartridge and earn rewards!
Have you used Simple Plex Mouse IL-12 p70 Cartridge?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Associated Pathways
Th1 Differentiation Pathway
