Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

R&D Systems | Catalog # SPCKB-MP-003636

Simple Plex Mouse/Rat Angiopoietin-2/ANG-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.1 pg/mL

Assay Range

10.5-40130 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse/Rat Angiopoietin-2 Cartridge Summary

Simple Plex assay is for the detection of mouse and rat Angiopoietin-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Angiopoietin-2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse, Rat

Specificity

Natural and recombinant mouse and rat Angiopoietin-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Angiopoietin-2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Angiopoietin-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for Angiopoietin-2.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 93 89
Mean (pg/mL) 198 10863 240 11457
Standard Deviation 18.6 452 27.5 1311
CV% 9.4 4.2 11.5 11.4

Recovery for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

Recovery was not necessary as mouse/rat Angiopoietin-2 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Angiopoietin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Mouse/Rat Angiopoietin-2 Assay Linearity

Scientific Data Images for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

Simple Plex Mouse/Rat Angiopoietin-2 Assay Standard Curve

Simple Plex Mouse/Rat Angiopoietin-2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat Angiopoietin-2 Assay. Assay range is 10.5-40,130 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that regulates vascular remodeling by promoting endothelial cell survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation. Its direct interaction with Integrins promotes tumor cell invasion. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It associates into dimers, trimers, and larger multimers and binds the receptor Tie-2, with its function as an agonist or antagonist depending on its state of multimerization.

Alternate Names

ANGPT2

Entrez Gene IDs

285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)

Gene Symbol

ANGPT2

Additional Angiopoietin-2 Products

Product Documents for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

For research use only

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