Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

R&D Systems | Catalog # SPCKB-MP-008843

Simple Plex Mouse/Rat Chitinase 3-like 1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (10 µL), Rat Serum (10 µL), Mouse EDTA Plasma (10 µL), Rat EDTA Plasma (10 µL), Mouse Heparin Plasma (10 µL), Rat Heparin Plasma (10 µL), Mouse Cerebrospinal Fluid (10 µL), Mouse Bronchoalveolar Lavage (10 µL)

Sensitivity

1.08 pg/mL

Assay Range

4.06-15502 pg/mL (Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Cerebrospinal Fluid)

Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge Summary

Simple Plex assay for the detection of mouse and rat Chitinase 3-like 1 (CHI3L1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin), bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Chitinase 3-like 1/YKL-40 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse, Rat

Specificity

Natural and recombinant mouse and rat CHI3L1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Mouse Bronchoalveolar Lavage, Mouse Cerebrospinal Fluid, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum, Rat Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 41 43
Mean (pg/mL) 116 559 101 5135
Standard Deviation 3.64 439 10.8 469
CV% 3.1 7.8 10.7 9.1

Recovery for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 116 115-117

Linearity

Scientific Data Images for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

Mouse/Rat CH3L1 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1/YKL-40 (CHI3L1), also called BRP39 in mouse, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Alternate Names

CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Gene Symbol

CHI3L1

Additional Chitinase 3-like 1/YKL-40 Products

Product Documents for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

For research use only

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