Simple Plex Mouse/Rat Cystatin C Cartridge

Catalog #: SPCKB-MP-005830 Datasheet / COA / SDS

Simple Plex Cartridge Kit for quantitation of Cystatin C. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-005830
SPCKA-MP-005830
SPCKC-MP-005830

Control Products Available

898389: Simple Plex Control for Mouse/Rat Cystatin C

Simple Plex Cartridge Kit for 72 samples, containing CyC/Cystatin C

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat Cystatin C Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (25 nL), Rat Serum (25 nL), Mouse EDTA Plasma (25 nL), Rat EDTA Plasma (25 nL), Urine (25 nL)

Sensitivity

0.27 pg/mL

Assay Range

0.9 - 3,590 pg/mL (Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Urine)

Specificity

Natural and recombinant mouse/rat Cystatin C.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse/rat Cystatin C in serum, EDTA plasma, and urine

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	19
Mean (pg/mL)	20.7	991	18.7	885
Standard Deviation	1.26	90.7	1.4	88.9
CV%	6.1	9.1	7.5	10

Recovery

Recovery was not necessary as mouse/rat Cystatin C shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Cystatin C were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Rat Cystatin C Simple Plex Assay Linearity

Scientific Data

N/A

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Mouse/Rat Cystatin C Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cystatin C

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

Entrez Gene IDs:

1471 (Human); 13010 (Mouse); 25307 (Rat)

Alternate Names:

ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin