Simple Plex Mouse/Rat Cystatin C Cartridge

Catalog #: SPCKB-MP-005830 Datasheet / COA / SDS
Simple Plex Cartridge Kit for quantitation of Cystatin C. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system
Catalog # Availability Size / Price Qty
Control Products Available
Simple Plex Cartridge Kit for 72 samples, containing CyC/Cystatin C
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Product Details
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Simple Plex Mouse/Rat Cystatin C Cartridge Summary

Sample Type & Volume Required Per Well*
Mouse Serum (25 nL), Rat Serum (25 nL), Mouse EDTA Plasma (25 nL), Rat EDTA Plasma (25 nL), Urine (25 nL)
0.27 pg/mL
Assay Range
0.9 - 3,590 pg/mL (Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Urine)
Natural and recombinant mouse/rat Cystatin C.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse/rat Cystatin C in serum, EDTA plasma, and urine


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 19
Mean (pg/mL) 20.7 991 18.7 885
Standard Deviation 1.26 90.7 1.4 88.9
CV% 6.1 9.1 7.5 10


Recovery was not necessary as mouse/rat Cystatin C shows natural linearity data from a neat sample to a 1:16 dilution.


Samples containing and/or spiked with high concentrations of mouse/rat Cystatin C were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Mouse Cystatin C Simple Plex Assay Linearity
Rat Cystatin C Simple Plex Assay Linearity

Scientific Data

Mouse/Rat Cystatin C Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cystatin C

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

Entrez Gene IDs:
1471 (Human); 13010 (Mouse); 25307 (Rat)
Alternate Names:
ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin


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