Simple Plex Mouse/Rat SP-D Cartridge

Catalog #: SPCKB-MP-008605 Datasheet / COA / SDS
Simple Plex Mouse/Rat SP-D assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-MP-008605
SPCKA-MP-008605
SPCKC-MP-008605
Control Products Available
Mouse/Rat SP-D Ella Assay
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Simple Plex Mouse/Rat SP-D Cartridge Summary

Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Mouse Bronchoalveolar Lavage (10 uL), Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
1.8 pg/mL
Assay Range
7.1 - 26,909 pg/mL (Bronchoalveolar Lavage Fluid, Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat SP-D.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat Surfactant Protein D (SP-D) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) and Bronchoalveolar Lavage Fluid (BALF)*.
*BALF are validated for mouse samples only.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bronchoalveolar Lavage Fluid, Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 44 42
Mean (pg/mL) 187 9028 201 9644
Standard Deviation 13.8 826 25.3 1132
CV% 7.4 9.2 12.6 11.7

Recovery

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 92 82-102

Linearity

Samples containing and/or spiked with high concentrations of SP-D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Mouse SP-D Ella Assay Linearity
Rat SP-D Ella Assay Linearity

Scientific Data

Mouse/Rat SP-D Ella Assay Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Long Name:
Surfactant Pulmonary Associated Protein D
Entrez Gene IDs:
6441 (Human); 20390 (Mouse)
Alternate Names:
COLEC7collectin-7; Collectin 7; Collectin-7; Lung surfactant protein D; PSPD; PSP-D; SFTP4; SFTP4pulmonary surfactant-associated protein D; SFTPD; SPD; SP-D; SP-Dpulmonary surfactant apoprotein; surfactant protein D; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4

FAQs

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