Simple Plex Non-Human Primate TREM2 Cartridge

R&D Systems | Catalog # SPCKB-NP-016628

Simple Plex Non-Human Primate TREM2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

1.78 pg/mL

Assay Range

9.96-15200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Non-Human Primate TREM2 Cartridge Summary

Simple Plex assay is for the detection of non-human primate TREM2 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TREM2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Non-human Primate

Specificity

Natural and recombinant TREM2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TREM2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each TREM2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for non-human primate TREM2.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 17 16
Mean (pg/mL) 98.6 4434 86.5 4250
Standard Deviation 5.10 136 8.20 392
CV% 5.2 3.1 9.5 9.2

Recovery for Simple Plex Non-Human Primate TREM2 Cartridge

Recovery for the non-human primate TREM2 assays was evaluated at three different spiked concentrations of TREM2 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 118 117-118

Linearity

Samples containing and/or spiked with high concentrations of TREM2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Non-Human Primate TREM2 Assay Linearity

Scientific Data Images for Simple Plex Non-Human Primate TREM2 Cartridge

Simple Plex Non-Human Primate TREM2 Assay Standard Curve

Simple Plex Non-Human Primate TREM2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Non-Human Primate TREM2 Assay. Assay range is 9.96-15,200 pg/mL.

Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells 2) is a transmembrane protein that is expressed on newly differentiated and alternatively activated tissue macrophages, immature myeloid dendritic cells, and osteoclasts. TREM2 interacts with and modifies signaling through dendritic cell and osteoclast Plexin A1, a semaphorin receptor. It is active in several settings that involve membrane fusion, including phagocytosis of microbes by macrophages and apoptotic neurons by microglia, and formation of osteoclasts and multinucleated giant cells from macrophages. TREM2 signals through the adaptor protein DAP12 and can associate with the linker protein LAT2 to inhibit macrophage activaation. A soluble isoform of TREM2 antagonizes full-length TREM2 and is elevated in cerebrospinal fluid of patients with active multiple sclerosis.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

Additional TREM2 Products

Product Documents for Simple Plex Non-Human Primate TREM2 Cartridge

Certificate of Analysis

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Product Specific Notices for Simple Plex Non-Human Primate TREM2 Cartridge

For research use only

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