Simple Plex Protein A Cartridge

R&D Systems | Catalog # SPCKB-OT-009204

Simple Plex Protein A assay kit contains cartridge, sample diluent SD62, and wash buffer.
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bioprocess Samples (25 µL)

Sensitivity

4.34 pg/mL

Assay Range

11.5-17500 pg/mL (Bioprocess Samples)

Simple Plex Protein A Cartridge Summary

Simple Plex assay for the detection of natural and engineered variant Protein A in Bioprocess samples. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Protein A Simple Plex »

Compatible Instruments

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Ella Automated Immunoassay System

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Specificity

Natural and engineered variant Protein A in Bioprocess samples. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each Protein A control was tested multiple times in one Protein A assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 29 27
Mean (pg/mL) 216 8267 167 7065
Standard Deviation 4.60 234 19.8 642
CV% 2.1 2.8 11.8 9.1

Recovery for Simple Plex Protein A Cartridge

Recovery at four different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Bioprocess Samples (n=6) 97 90-103

Linearity

Samples containing and/or spiked with high concentrations Protein A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Protein A Assay Linearity

Scientific Data Images for Simple Plex Protein A Cartridge

Simple Plex Protein A Assay Standard Curve

Simple Plex Protein A Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Protein A Assay. Assay range is 11.50-17,500 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Protein A

Protein A is a Staphylococcus aureus cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially those of the IgG class. Because of this property, it is often utilized for microscopic visualization of molecules and to purify antisera. Protein A has also been used for the treatment of proteinuria in nephrotic syndrome, and severe autoimmune diseases such as rheumatoid arthritis, coeliac disease, and systemic lupus erythematosis.

Alternate Names

PROA, Protein-A

Entrez Gene IDs

3919448 (S. aureus)

Gene Symbol

SAOUHSC_00069

Additional Protein A Products

Product Documents for Simple Plex Protein A Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Protein A Cartridge

For research use only

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