Simple Plex Rat Lipocalin-2/NGAL Cartridge

Catalog #: SPCKB-RP-000546 Datasheet / COA / SDS
Simple Plex Cartridge Kit for quantitation of Lipocalin-2. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system
Catalog # Availability Size / Price Qty
Control Products Available
Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Rat Plasma / Serum.
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Product Details
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Simple Plex Rat Lipocalin-2/NGAL Cartridge Summary

Sample Type & Volume Required Per Well*
Rat Serum (25 nL), Rat EDTA Plasma (25 nL), Rat Urine (25 nL)
2.62 Not Assigned
Assay Range
2.6 - 10,000 Not Assigned (Rat Serum, Rat EDTA Plasma, Rat Urine)
Natural and recombinant rat Lipocalin-2/NGAL
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of rat Lipocalin-2 in serum, plasma (EDTA), and urine


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Rat Serum, Rat EDTA Plasma, Rat Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4
n 16 16 16 16
Mean (pg/mL) 48.7 2552 51.5 2683
Standard Deviation 1.87 249 3.88 230
CV% 3.8 9.8 7.5 8.6


Samples containing and/or spiked with high concentrations of rat Lipocalin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Rat Lipocalin-2/NGAL Simple Plex Assay Linearity

Scientific Data

Rat Lipocalin-2/NGAL Simple Plex Assay Standard Curve

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs:
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names:
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin


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