Simple Plex Rat Lipocalin-2/NGAL Cartridge

R&D Systems | Catalog # SPCKB-RP-000546

Simple Plex Cartridge Kit for quantitation of Lipocalin-2. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Urine (10 µL)

Sensitivity

0.69 pg/mL

Assay Range

2.62-10000 pg/mL (Serum, Rat Serum, EDTA Plasma, Rat EDTA Plasma, Urine, Rat Urine)

Simple Plex Rat Lipocalin-2/NGAL Cartridge Summary

Simple Plex assay is for the detection of rat Lipocalin-2 in serum, EDTA plasma, and urine in Sample Diluent SD09. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all MMP-9 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Rat

Specificity

Natural and recombinant rat Lipocalin-2/NGAL.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Lipocalin-2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Lipocalin-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for rat Lipocalin-2.

EDTA Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 19
Mean (pg/mL) 48.7 2552 51.5 2683
Standard Deviation 1.87 249 3.88 230
CV% 3.8 9.8 7.5 8.6

Recovery for Simple Plex Rat Lipocalin-2/NGAL Cartridge

Recovery was not necessary as rat Lipocalin-2 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of rat Lipocalin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Rat Lipocalin-2/NGAL Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Rat Lipocalin-2/NGAL Cartridge

Simple Plex Mouse/Rat Lipocalin-2/NGAL Assay Standard Curve

Simple Plex Mouse/Rat Lipocalin-2/NGAL Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Rat Lipocalin-2/NGAL Assay. Assay range is 2.62-10,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

MMP-9 (Gelatinase B) is a metalloproteinase that degrades multiple extracellular matrix components including Gelatin, Collagens III, IV, V, and XI, Elastin, Nidogen-1, and Vitronectin. It also cleaves a variety of chemokines and growth factors (e.g. IL-1 beta, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta, membrane bound TNF-alpha, VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein. MMP-9 can trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and CD25/IL-2 R alpha). The activity of MMP-9 is regulated by its association with the TIMP-1 protease inhibitor. Circulating levels of MMP-9 are elevated in many inflammatory disorders including intraluminal thrombus formation, atherosclerosis, Crohn’s disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for Simple Plex Rat Lipocalin-2/NGAL Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Rat Lipocalin-2/NGAL Cartridge

For research use only

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